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| Registros recuperados: 13 | |
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| Silva,D.G.; Silva,P.R.L.; Fagliari,J.J.; Ávila,F.A; Alessi,A.C.; Oliveira,R.G.. |
| The clinical conditions of healthy calves infected with experimental 10(8)CFU of Salmonella Dublin were evaluated and the viability of the experimental model in disease induction in calves was verified. Twelve 10 to 15-day-old male Holstein calves were examined. They were allocated into two groups, control and experimentally infected with 10(8)CFU of Salmonella Dublin. Animals were submitted to clinical examination after inoculation and at every 12 hours, during seven days after the experimental infection. Samples of rectal swabs were collected for Salmonella Dublin isolation. All animals had severe diarrhea, with mucus and bleeding, 12 to 84 hours after the experimental infection with Salmonella Dublin, accompanied by fever, dehydration and respiratory... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bezerro; Salmonella Dublin; Salmonelose. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000100034 |
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| Ávila,L.G.; Silva,D.G.; Sato,R.A.; Fagliari,J.J.. |
| The clinical conditions of healthy calves infected with 10(9) CFU of Salmonella Typhimurium were evaluated and the viability of the experimental model in disease induction was verified. Twelve male Holstein calves, 10 to 15-days old, were examined. They were allocated into two groups, control and experimentally infected. Animals were submitted to clinical examination after inoculation and at every 24 hours, during six days after infection. Samples of rectal swabs were collected for Salmonella Typhimurium isolation. Results showed that all animals had different degrees of diarrhea, with mucus and bleeding, 24 to 72 hours after experimental infection with Salmonella Typhimurium, accompanied by fever, but no signs of sepsis. The isolation of Salmonella... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calf; Salmonellosis; Diarrhea. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600043 |
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| Silva,D.G.; Montassier,H.J.; Oliveira,R.G.; Fuentes,D.B.; Samara,S.I.; Jerez,J.A.; Buzinaro,M.G.. |
| Com o objetivo de monitorar a imunidade passiva em bezerros alimentados com colostro de vacas imunizadas e não imunizadas com vacina contra rotavírus, foram determinados títulos de anticorpos em amostras de sangue e colostro de 26 vacas da raça Holandesa no dia do parto e de seus bezerros, à zero, às 24, 48 horas e aos sete, 14, 21, 28 dias de idade, pelo ensaio imunoenzimático. Tanto no soro sangüíneo como no colostro, os títulos dos isótipos IgG, IgG1 e IgG2 foram mais elevados no grupo dos animais vacinados, porém somente no colostro o aumento foi significativo. Os bezerros alimentados com o colostro das vacas vacinadas apresentaram títulos mais altos dos isótipos IgG, IgG1, IgG2, IgA e IgM, após a ingestão do colostro, sendo constatado aumento... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovino; Colostro; Imunidade passiva; Rotavírus; Vacina. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000500008 |
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| Silva,D.G.; Silva,D.R.; Silva,P.R.L.; Cícero,E.A.S.; Ferraz,A.L.J.; Lemos,M.V.F.; Fagliari,J.J.. |
| The efficiency of the Polymerase Chain Reaction (PCR) combined with selective enrichment broth was compared with the standard microbiological techniques for detection of Salmonella Dublin in fecal samples of 10 to 15-days-old Holstein calves, experimentally infected with 10(8) CFU of Salmonella Dublin. Seventy-six fecal samples were analyzed using PCR associated with selenite cystine (SC) and Muller-Kauffmann tetrathionate (TMK) broths and standard microbiological techniques. Regardless of the selective enrichment broth used, the standard microbiological techniques were significantly better than PCR in detection of positive samples of Salmonella Dublin. However, the simultaneous use of both techniques provided detection of a larger number of positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calves; Feces; Salmonella Dublin; PCR; Culture. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000300035 |
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| Silva,D.G.; Fagliari,J.J.; Garcia,T.B.. |
| The aim of this study was to compare three different selective enrichment broths: Rappaport-Vassiliadis (RV), selenite cystine (SC) and Muller-Kauffmann tetrathionate (MKT) for Salmonella Dublin isolation from faecal samples of calf experimentally infected. The bacteriological procedure involved pre-enrichment stages in Hajna-GN broth (only for the samples inoculated in RV broth), selective enrichment, culture in modified brilliant green agar (BGA), presumptive biochemistry tests (using triple-sugar-iron agar and lysine-agar) and slide agglutination test with poli-O and poli-H Salmonella antiserum. The effects of enrichment temperatures using RV broth were also evaluated (37ºC and 42ºC). SC broth was significantly more efficient in the isolation of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bezerro; Salmonella Dublin; Meio seletivo; Fezes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300036 |
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| Silva,D.G.; Silva,P.R.L.; Fagliari,J.J.. |
| O objetivo do estudo foi avaliar o hemograma e o perfil bioquímico sérico, inclusive hemogasométrico, de bezerros infectados experimentalmente com Salmonella Dublin. Foram utilizados 12 bezerros sadios da raça Holandesa com 10 a 15 dias de idade, distribuídos aleatoriamente em dois grupos experimentais: grupo-controle (n= 6) e grupo infectado com 10(8)UFC de Salmonella Dublin (n=6). Os bezerros foram submetidos ao exame físico diário, e as amostras de sangue foram coletadas minutos antes da inoculação (0h) e 24, 48, 72, 96, 120, 144 e 168h após a inoculação. Além do hemograma e das análises hemogasométricas, foram mensuradas as atividades séricas das enzimas aspartato aminotransferase (AST), fosfatase alcalina (ALP), creatina cinase (CK),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bezerro; Salmonella Dublin; Diarreia; Hemograma; Eletrólitos. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000200002 |
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| Pizauro,L.J.L.; Silva,D.G.; Santana,A.M.; Clemente,V.; Lara,G.H.B.; Listoni,F.J.P.; Vaz,A.C.N.; Vidal-Martins,A.M.C.; Ribeiro,M.G.; Fagliari,J.J.. |
| The aim of this study was to evaluate somatic cell count (SCC), prevalence and etiology of mastitis in a dairy buffalo herd from Analândia, São Paulo State, Brazil, in the dry and rainy seasons. Additionally, antimicrobial susceptibility profile of microorganisms isolated from milk samples was also evaluated. 1,042 milk samples from female Murrah buffaloes in a dairy farm located in Analândia, São Paulo State, Brazil, collected between May 2011 and November 2012 were analyzed. After the mammary gland physical examination, strip cup test and California Mastitis Test (CMT) were performed. Afterwards, 50mL of milk samples from each mammary quarter were collected aseptically for SCC in automatic equipment and microbiological examination. The antimicrobial... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antibiogram; Bubalus bubalis; SCC; Mammary gland; Microorganisms. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601703 |
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| Silva,D.G.; Santana,A.M.; Maluta,R.P.; Pizauro,L.J.L.; Bernardes,P.A.; Aquino,G.V.; Benincasa,N.C.; Rodrigues,D.P.; Fagliari,J.J.. |
| RESUMO O objetivo do estudo foi investigar a prevalência de Salmonella spp. em amostras de fezes de búfalos do estado de São Paulo, Brasil, e identificar os sorotipos isolados. Foram examinadas 116 amostras de suabes retais de búfalos das raças Jafarabadi e Murrah, coletadas em triplicata, em seis propriedades rurais localizadas nas regiões Central, Centro-Oeste e Nordeste do estado de São Paulo, Brasil. Para avaliar a presença de Salmonella spp., foram utilizados três diferentes caldos de enriquecimento (caldo selenito cistina, caldo tetrationado Muller-Kauffmann e caldo Rappaport-Vassiliadis) e dois diferentes meios de cultura (ágar verde brilhante modificado e ágar XLT4). Das 116 amostras de suabes retais examinadas, oito amostras (6,90%; 8/116) foram... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bubalus bubalis; Enrichment broth; Feces; Salmonella; Serotype. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501645 |
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| Fagliari,J.J.; Rizolli,F.W.; Silva,S.L.; Silva,D.G.. |
| The serum protein concentration of newborn Holstein calves determined by means of sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) was studied. Blood samples from 40 healthy newborn calves were obtained 48 hours after birth. Calves had been given 3 liters of colostrum within 2 hours after birth, following by dose corresponding by 15% of animal weight each 24 hours. The results showed three different proteinograms: 19 calves had 14 proteins with molecular weights (MW) ranging from 28,000 D to 170,000D (proteinogram 1); 11 calves had 14 proteins with MW ranging from 18,000 to 170,000 D (proteinogram 1); and 10 calves had 12 proteins with MW ranging from 28,000 D to 170,000 D (proteinogram 3). The three groups presented similar IgG levels. The highest serum... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bezerro; Proteinograma; Eletroforese; SDS-PAGE. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300026 |
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| Freitas,P.P.S.; Uyemura,S.A.; Silva,D.G.; Samara,S.I.; Buzinaro,M.G.. |
| O estudo de prevalência da infecção por rotavírus em bezerros abrangeu 51 rebanhos leiteiros, escolhidos ao acaso, localizados em uma região produtora de leite do estado de São Paulo. Entre 31 de maio e 20 de outubro de 2003, foram colhidas 103 amostras de fezes de bezerros com diarreia e 308 amostras de animais sem diarreia, com idade entre um e 45 dias. As amostras foram analisadas pelas técnicas de ensaio imunoenzimático (EIE) e eletroforese em gel de poliacrilamida (PAGE). Pelo EIE foi observada prevalência de rotavírus de 21,6% (11/51) nos rebanhos e 6,7% (27/404) nos bezerros. Foram diagnosticados animais infectados por rotavírus tanto em bezerros diarreicos (18,4%; 19/103) quanto em bezerros assintomáticos (2,7%; 8/301). A maior frequência de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bezerro; Rotavírus; Diarreia; Fatores de risco; Genotipagem. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400005 |
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| Souza,D.C.; Silva,D.G.; Rocha,T.G.; Monteiro,B.M.; Pereira,G.T.; Fiori,L.C.; Viana,R.B.; Fagliari,J.J.. |
| ABSTRACT Serum blood samples from 50 Murrah buffalo calves were examined in this study. The animals were allocated into three groups according to the number of parturitions of their mothers: G1 (n= 15) calves from primiparous buffaloes, G2 (n= 19) calves from buffaloes with two to four parturitions, and G3 (n= 16) calves from buffaloes with five or more parturitions. Blood samples were taken at birth, before colostrum ingestion, at 24h, 48h, and 72h after birth, and at 7, 14, 21, and 30 days after birth for determination of levels of gammaglutamyl transferase (GGT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), creatine kinase, total protein, albumin, globulins (including immunoglobulin G), iron, total calcium, ionized calcium, phosphorus,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bubalus bubalis; Newborn; Number of parturitions; Murrah; Passive immunity transfer (PIT). |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100187 |
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| Rocha,T.G.; Silva,F.D.F.; Bortoletto,C.; Silva,D.G.; Buzinaro,M.G.; Zafalon,L.F.; Fagliari,J.J.. |
| ABSTRACT The aim of the present study was to characterize changes in acute phase protein levels according to the occurrence of rotavirus diarrhea in calves in the first month of life. Blood and fecal samples were taken before colostrum intake and at 1, 2, 7, 15, 21 and 30 days of age from 24 Holstein calves allotted in three experimental groups: calves that did not present diarrhea (group A), calves that presented diarrhea, but tested negative for rotavirus in feces (group B), and calves that presented diarrhea and tested positive for rotavirus in feces (group C) (experiment 1). When the animals presented episodes of diarrhea, blood and fecal samples were taken at 24-hour intervals until the end of clinical signs (experiment 2). Serum proteins were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine rotavirus; Bovine neonatal diarrhea; Serum proteinogram; Ceruloplasmin. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000400865 |
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| Registros recuperados: 13 | |
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